Methods of DNA Sequencing

DNA sequencing is a process to decode the DNA in terms of its basic units, adenine, guanine, cytosine, and thymine. Now, DNA sequencing includes several methods and technologies that are used for determining the order of the nucleotide bases in a strand of DNA.


Difference in work flow of conventional versus second-generation sequencing

Nature Biotechnology 26, 1135 - 1145 (2008)
High-throughput sequencing methods
  • Lynx Therapeutics' Massively Parallel Signature Sequencing (MPSS)
  • Polony sequencing
  • 454 pyrosequencing
  • Illumina (Solexa) sequencing
  • SOLiD sequencing
  • Ion semiconductor sequencing
  • DNA nanoball sequencing
  • Helioscope(TM) single molecule sequencing
  • Single Molecule SMRT(TM) sequencing
  • Single Molecule real time (RNAP) sequencing
  • Nanopore DNA sequencing
  • VisiGen Biotechnologies approach



First Generation:











Second Generation:
Pyrosequencing Entry: 







Helicose Entry:







454 Entry






Third Generation:







Further reading:
  1.  Next-generation DNA sequencingNat. Biotechnol.2008;26:1135-1145  Abstract

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