Sequence Similarity Search - I : Basic Local Alignment Search Tool (BLAST)
Basic Local Alignment Search Tool (BLAST) is a bioinformatics Tool to finds regions of local similarity between sequences. BLAST compares biological sequence information, nucleotide or protein sequences, to sequence databases and calculates the statistical significance of matches and thus helps us to infer functional and evolutionary relationships between sequences. Different types of BLASTs are available according to the query sequences. BLAST is actually a family of programs where every member perform specific function
blastp amino acid query Vs a protein sequence database
blastn nucleotide query sequence Vs nucleotide sequence database
blastx nucleotide query sequence translated in all reading frames Vs protein sequence database
tblastn protein query sequence Vs nucleotide sequence database
tblastx six-frame translatied nucleotide query sequence Vs six-frame translations of a nucleotide sequence database
Beside the above mentioned BLAST type some relatively new BLAST algorithms have been developed by different scientific groups to deals efficiently with sequences. Some examples are
Position-Specific Iterative BLAST (PSI-BLAST) - is used to find distant relatives of a protein because of its high sensitivity in picking up distant evolutionary relationships than a standard protein-protein BLAST.
Megablast - for comparision of large numbers of query sequences
Discontiguous megablast - for comparison of large set of diverged sequencesAmong these BLAST programs, BLASTn and BLASTp are the most commonly used because they use direct comparisons of sequences.
Further reading :1. Blast Program Selection Guide HERE
2. Discontiguous Mega BLAST HERE
3. BLAST tutorial HERE
NEXT POST : Blast VIDEO TUTORIAL
First of all, let me clear it that i am not a Bioinformatician. i am simple plant biology researcher who face problem in her daily research life and who bother to post solution of those problem on this webpage. That's it.